Science · 2018

CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity

Chen JS, Ma E, Harrington LB, Da Costa M, Tian X, Palefsky JM, Doudna JA.

Show affiliations
  • Chen JSDepartment of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA.
  • Ma EDepartment of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA.
  • Harrington LBDepartment of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA.
  • Da Costa MDepartment of Medicine, University of California, San Francisco, San Francisco, CA 94143, USA.
  • Tian XDepartment of Chemistry, University of California, Berkeley, Berkeley, CA 94720, USA.
  • Palefsky JMDepartment of Medicine, University of California, San Francisco, San Francisco, CA 94143, USA.
  • Doudna JADepartment of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA. doudna@berkeley.edu.
31 read8 want to readRigor 3.1Novelty 3.2Impact 2.7Reprod. 3.0

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0

Ines Sato

Rigor 2/5Novelty 5/5Impact 5/5Reprod. 3/5

Great figures and clear story.

0

Lena Brown

Rigor 2/5Novelty 2/5Impact 1/5Reprod. 1/5

Great figures and clear story.

0

Sara Rossi

Rigor 2/5Novelty 5/5Impact 5/5Reprod. 1/5

Important contribution to the field.

0

Sara Kim

Rigor 5/5Novelty 4/5Impact 4/5Reprod. 1/5

Solid methods, convincing results.

0

Pablo Wang

Rigor 2/5Novelty 2/5Impact 3/5Reprod. 1/5

Important contribution to the field.

0

Eva Garcia

Rigor 5/5Novelty 1/5Impact 1/5Reprod. 5/5

Nice but incremental.

0

Noor Patel

Rigor 3/5Novelty 1/5Impact 1/5Reprod. 5/5

Solid methods, convincing results.

0

Sara Patel

Rigor 4/5Novelty 1/5Impact 3/5Reprod. 4/5

Important contribution to the field.

0

Omar Lee

Rigor 3/5Novelty 3/5Impact 1/5Reprod. 3/5

Replication is unclear to me.

0

Jonas Smith

Rigor 4/5Novelty 3/5Impact 1/5Reprod. 5/5

Replication is unclear to me.

0

Tariq Brown

Rigor 3/5Novelty 4/5Impact 1/5Reprod. 3/5

Important contribution to the field.

0

Ines Nguyen

Rigor 1/5Novelty 4/5Impact 2/5Reprod. 5/5

Nice but incremental.

0

Jonas Rossi

Rigor 3/5Novelty 5/5Impact 1/5Reprod. 1/5

Nice but incremental.