Mol Plant · 2015

A Robust CRISPR/Cas9 System for Convenient, High-Efficiency Multiplex Genome Editing in Monocot and Dicot Plants

Ma X, Zhang Q, Zhu Q, Liu W, Chen Y, Qiu R, Wang B, Yang Z, Li H, Lin Y, Xie Y, Shen R, Chen S, Wang Z, Chen Y, Guo J, Chen L, Zhao X, Dong Z, Liu YG.

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  • Ma XState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangzhou 510642, China; College of Life Sciences, South China Agricultural University, Guangzhou 510642, China.
  • Zhang QState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangzhou 510642, China; Key Laboratory of Plant Functional Genomics and Biotechnology of Guangdong Provincial Higher Education Institutions, Guangzhou 510642, China; College of Life Sciences, South China Agricultural University, Guangzhou 510642, China.
  • Zhu QKey Laboratory of Plant Functional Genomics and Biotechnology of Guangdong Provincial Higher Education Institutions, Guangzhou 510642, China; College of Life Sciences, South China Agricultural University, Guangzhou 510642, China.
  • Liu WState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangzhou 510642, China; Key Laboratory of Plant Functional Genomics and Biotechnology of Guangdong Provincial Higher Education Institutions, Guangzhou 510642, China; College of Life Sciences, South China Agricultural University, Guangzhou 510642, China.
  • Chen YKey Laboratory of South China Agriculture Plant Molecular Analysis and Genetic Improvement, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China.
  • Qiu RState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangzhou 510642, China; Key Laboratory of Plant Functional Genomics and Biotechnology of Guangdong Provincial Higher Education Institutions, Guangzhou 510642, China; College of Life Sciences, South China Agricultural University, Guangzhou 510642, China.
  • Wang BState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangzhou 510642, China; Key Laboratory of Plant Functional Genomics and Biotechnology of Guangdong Provincial Higher Education Institutions, Guangzhou 510642, China; College of Life Sciences, South China Agricultural University, Guangzhou 510642, China.
  • Yang ZState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangzhou 510642, China; Key Laboratory of Plant Functional Genomics and Biotechnology of Guangdong Provincial Higher Education Institutions, Guangzhou 510642, China; College of Life Sciences, South China Agricultural University, Guangzhou 510642, China.
  • Li HState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangzhou 510642, China; Key Laboratory of Plant Functional Genomics and Biotechnology of Guangdong Provincial Higher Education Institutions, Guangzhou 510642, China; College of Life Sciences, South China Agricultural University, Guangzhou 510642, China.
  • Lin YState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangzhou 510642, China; Key Laboratory of Plant Functional Genomics and Biotechnology of Guangdong Provincial Higher Education Institutions, Guangzhou 510642, China; College of Life Sciences, South China Agricultural University, Guangzhou 510642, China.
  • Xie YState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangzhou 510642, China; Key Laboratory of Plant Functional Genomics and Biotechnology of Guangdong Provincial Higher Education Institutions, Guangzhou 510642, China; College of Life Sciences, South China Agricultural University, Guangzhou 510642, China.
  • Shen RState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangzhou 510642, China; Key Laboratory of Plant Functional Genomics and Biotechnology of Guangdong Provincial Higher Education Institutions, Guangzhou 510642, China; College of Life Sciences, South China Agricultural University, Guangzhou 510642, China.
  • Chen SState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangzhou 510642, China; Key Laboratory of Plant Functional Genomics and Biotechnology of Guangdong Provincial Higher Education Institutions, Guangzhou 510642, China; College of Life Sciences, South China Agricultural University, Guangzhou 510642, China.
  • Wang ZCollege of Life Sciences, South China Agricultural University, Guangzhou 510642, China.
  • Chen YState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangzhou 510642, China; Key Laboratory of Plant Functional Genomics and Biotechnology of Guangdong Provincial Higher Education Institutions, Guangzhou 510642, China; College of Life Sciences, South China Agricultural University, Guangzhou 510642, China.
  • Guo JState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangzhou 510642, China; Key Laboratory of Plant Functional Genomics and Biotechnology of Guangdong Provincial Higher Education Institutions, Guangzhou 510642, China; College of Life Sciences, South China Agricultural University, Guangzhou 510642, China.
  • Chen LState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangzhou 510642, China; Key Laboratory of Plant Functional Genomics and Biotechnology of Guangdong Provincial Higher Education Institutions, Guangzhou 510642, China; Guangdong Provincial Key Laboratory of Protein Function and Regulation in Agricultural Organisms, Guangzhou 510642, China; College of Life Sciences, South China Agricultural University, Guangzhou 510642, China.
  • Zhao XState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangzhou 510642, China; Key Laboratory of Plant Functional Genomics and Biotechnology of Guangdong Provincial Higher Education Institutions, Guangzhou 510642, China; College of Life Sciences, South China Agricultural University, Guangzhou 510642, China.
  • Dong ZKey Laboratory of South China Agriculture Plant Molecular Analysis and Genetic Improvement, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China.
  • Liu YGState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangzhou 510642, China; Key Laboratory of Plant Functional Genomics and Biotechnology of Guangdong Provincial Higher Education Institutions, Guangzhou 510642, China; College of Life Sciences, South China Agricultural University, Guangzhou 510642, China. Electronic address: ygliu@scau.edu.cn.
48 read8 want to readRigor 2.9Novelty 2.6Impact 3.0Reprod. 3.0

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0

Jonas Brown

Rigor 1/5Novelty 3/5Impact 4/5Reprod. 1/5

Great figures and clear story.

0

Maya Khan

Rigor 4/5Novelty 3/5Impact 3/5Reprod. 2/5

Nice but incremental.

0

Ines Khan

Rigor 3/5Novelty 4/5Impact 3/5Reprod. 1/5

Great figures and clear story.

0

Ines Cohen

Rigor 4/5Novelty 3/5Impact 2/5Reprod. 4/5

Interesting idea, small sample.

0

Yuki Mueller

Rigor 2/5Novelty 2/5Impact 4/5Reprod. 2/5

Great figures and clear story.

0

Ravi Mueller

Rigor 2/5Novelty 2/5Impact 3/5Reprod. 3/5

Great figures and clear story.

0

Sam Garcia

Rigor 4/5Novelty 1/5Impact 2/5Reprod. 4/5

Interesting idea, small sample.

0

Tom Patel

Rigor 4/5Novelty 2/5Impact 3/5Reprod. 2/5

Replication is unclear to me.

0

Tom Smith

Rigor 4/5Novelty 3/5Impact 5/5Reprod. 4/5

Great figures and clear story.

0

Liam Khan

Rigor 3/5Novelty 2/5Impact 2/5Reprod. 3/5

Important contribution to the field.