Nature · 2016

High-fidelity CRISPR-Cas9 nucleases with no detectable genome-wide off-target effects

Kleinstiver BP, Pattanayak V, Prew MS, Tsai SQ, Nguyen NT, Zheng Z, Joung JK.

Show affiliations
  • Kleinstiver BP — Molecular Pathology Unit, Center for Cancer Research, and Center for Computational and Integrative Biology, Massachusetts General Hospital, Charlestown, Massachusetts 02129, USA.
  • Pattanayak V — Molecular Pathology Unit, Center for Cancer Research, and Center for Computational and Integrative Biology, Massachusetts General Hospital, Charlestown, Massachusetts 02129, USA.
  • Prew MS — Molecular Pathology Unit, Center for Cancer Research, and Center for Computational and Integrative Biology, Massachusetts General Hospital, Charlestown, Massachusetts 02129, USA.
  • Tsai SQ — Molecular Pathology Unit, Center for Cancer Research, and Center for Computational and Integrative Biology, Massachusetts General Hospital, Charlestown, Massachusetts 02129, USA.
  • Nguyen NT — Molecular Pathology Unit, Center for Cancer Research, and Center for Computational and Integrative Biology, Massachusetts General Hospital, Charlestown, Massachusetts 02129, USA.
  • Zheng Z — Department of Biomedical Sciences, City University of Hong Kong, Hong Kong, China.
  • Joung JK — Molecular Pathology Unit, Center for Cancer Research, and Center for Computational and Integrative Biology, Massachusetts General Hospital, Charlestown, Massachusetts 02129, USA.
20 read2 want to readRigor 2.6Novelty 3.3Impact 2.6Reprod. 3.4

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0

Omar Khan

Rigor 1/5Novelty 5/5Impact 2/5Reprod. 5/5

Replication is unclear to me.

0

Liam Kim

Rigor 3/5Novelty 4/5Impact 2/5Reprod. 3/5

Great figures and clear story.

0

Tom Patel

Rigor 3/5Novelty 3/5Impact 4/5Reprod. 2/5

Important contribution to the field.