Nat Biotechnol · 2015

GUIDE-seq enables genome-wide profiling of off-target cleavage by CRISPR-Cas nucleases

Tsai SQ, Zheng Z, Nguyen NT, Liebers M, Topkar VV, Thapar V, Wyvekens N, Khayter C, Iafrate AJ, Le LP, Aryee MJ, Joung JK.

Show affiliations
  • Tsai SQ — Molecular Pathology Unit, Massachusetts General Hospital, Charlestown, MA USA.
  • Zheng Z — Molecular Pathology Unit, Massachusetts General Hospital, Charlestown, MA USA.
  • Nguyen NT — Molecular Pathology Unit, Massachusetts General Hospital, Charlestown, MA USA.
  • Liebers M — Molecular Pathology Unit, Massachusetts General Hospital, Charlestown, MA USA.
  • Topkar VV — Molecular Pathology Unit, Massachusetts General Hospital, Charlestown, MA USA.
  • Thapar V — Molecular Pathology Unit, Massachusetts General Hospital, Charlestown, MA USA.
  • Wyvekens N — Molecular Pathology Unit, Massachusetts General Hospital, Charlestown, MA USA.
  • Khayter C — Molecular Pathology Unit, Massachusetts General Hospital, Charlestown, MA USA.
  • Iafrate AJ — Molecular Pathology Unit, Massachusetts General Hospital, Charlestown, MA USA.
  • Le LP — Molecular Pathology Unit, Massachusetts General Hospital, Charlestown, MA USA.
  • Aryee MJ — Molecular Pathology Unit, Massachusetts General Hospital, Charlestown, MA USA.
  • Joung JK — Molecular Pathology Unit, Massachusetts General Hospital, Charlestown, MA USA.
78 read8 want to readRigor 2.8Novelty 2.8Impact 3.1Reprod. 2.7

Most-cited papers citing this

  • Nature · 2026 · cited by 13
  • Nat Biotechnol · 2026 · cited by 5
  • G3 (Bethesda) · 2026 · cited by 4
  • Nature · 2026 · cited by 3
  • Nature · 2026 · cited by 3

Log in to like, log, and share thoughts on this paper.

Discussion

Share your thoughts above to start a thread. Constructive feedback only — reply to and like others’ posts.

0

Alex Sato

Rigor 1/5Novelty 3/5Impact 5/5Reprod. 3/5

Important contribution to the field.

0

Omar Brown

Rigor 2/5Novelty 1/5Impact 3/5Reprod. 4/5

Important contribution to the field.

0

Sam Khan

Rigor 5/5Novelty 5/5Impact 3/5Reprod. 4/5

Interesting idea, small sample.

0

Sara Nguyen

Rigor 5/5Novelty 3/5Impact 5/5Reprod. 2/5

Important contribution to the field.

0

Alex Kim

Rigor 2/5Novelty 2/5Impact 5/5Reprod. 4/5

Nice but incremental.

0

Liam Kim

Rigor 1/5Novelty 2/5Impact 2/5Reprod. 1/5

Nice but incremental.

0

Sam Mueller

Rigor 2/5Novelty 1/5Impact 4/5Reprod. 1/5

Interesting idea, small sample.

0

Tariq Wang

Rigor 5/5Novelty 1/5Impact 3/5Reprod. 1/5

Solid methods, convincing results.

0

Omar Patel

Rigor 2/5Novelty 3/5Impact 2/5Reprod. 2/5

Important contribution to the field.

0

Sam Sato

Rigor 3/5Novelty 4/5Impact 1/5Reprod. 1/5

Great figures and clear story.

0

Ken Khan

Rigor 2/5Novelty 2/5Impact 3/5Reprod. 5/5

Great figures and clear story.

0

Ravi Nguyen

Rigor 4/5Novelty 5/5Impact 1/5Reprod. 3/5

Solid methods, convincing results.

0

Liam Rossi

Rigor 2/5Novelty 2/5Impact 4/5Reprod. 3/5

Replication is unclear to me.

0

Ravi Kim

Rigor 2/5Novelty 1/5Impact 3/5Reprod. 5/5

Nice but incremental.

0

Eva Wang

Rigor 4/5Novelty 3/5Impact 2/5Reprod. 5/5

Interesting idea, small sample.

0

Ines Patel

Rigor 2/5Novelty 3/5Impact 3/5Reprod. 3/5

Important contribution to the field.

0

Liam Smith

Rigor 2/5Novelty 5/5Impact 1/5Reprod. 2/5

Great figures and clear story.

0

Omar Smith

Rigor 3/5Novelty 2/5Impact 4/5Reprod. 4/5

Nice but incremental.