Nat Rev Microbiol · 2020

Evolutionary classification of CRISPR-Cas systems: a burst of class 2 and derived variants

Makarova KS, Wolf YI, Iranzo J, Shmakov SA, Alkhnbashi OS, Brouns SJJ, Charpentier E, Cheng D, Haft DH, Horvath P, Moineau S, Mojica FJM, Scott D, Shah SA, Siksnys V, Terns MP, Venclovas Č, White MF, Yakunin AF, Yan W, Zhang F, Garrett RA, Backofen R, van der Oost J, Barrangou R, Koonin EV.

Show affiliations
  • Makarova KSNational Center for Biotechnology Information, National Library of Medicine, Bethesda, MD, USA.
  • Wolf YINational Center for Biotechnology Information, National Library of Medicine, Bethesda, MD, USA.
  • Iranzo JNational Center for Biotechnology Information, National Library of Medicine, Bethesda, MD, USA.
  • Shmakov SANational Center for Biotechnology Information, National Library of Medicine, Bethesda, MD, USA.
  • Alkhnbashi OSBioinformatics group, Department of Computer Science, University of Freiberg, Freiberg, Germany.
  • Brouns SJJKavli Institute of Nanoscience, Department of Bionanoscience, Delft University of Technology, Delft, The Netherlands.
  • Charpentier EMax Planck Unit for the Science of Pathogens, Humboldt University, Berlin, Germany.
  • Cheng DArbor Biotechnologies, Cambridge, MA, USA.
  • Haft DHNational Center for Biotechnology Information, National Library of Medicine, Bethesda, MD, USA.
  • Horvath PDuPont Nutrition and Health, Dangé-Saint-Romain, France.
  • Moineau SDépartement de biochimie, de microbiologie et de bio-informatique, Faculté des sciences et de génie, Groupe de recherche en écologie buccale, Félix d'Hérelle Reference Center for Bacterial Viruses, Faculté de médecine dentaire, Université Laval, Québec City, Québec, Canada.
  • Mojica FJMDepartamento de Fisiología, Genética y Microbiología, Universidad de Alicante, Alicante, Spain.
  • Scott DArbor Biotechnologies, Cambridge, MA, USA.
  • Shah SACOPSAC, Copenhagen Prospective Studies on Asthma in Childhood, Herlev and Gentofte Hospital, University of Copenhagen, Gentofte, Denmark.
  • Siksnys VInstitute of Biotechnology, Life Sciences Center, Vilnius University, Vilnius, Lithuania.
  • Terns MPBiochemistry and Molecular Biology, Genetics and Microbiology, University of Georgia, Athens, GA, USA.
  • Venclovas ČInstitute of Biotechnology, Life Sciences Center, Vilnius University, Vilnius, Lithuania.
  • White MFBiomedical Sciences Research Complex, University of St. Andrews, St. Andrews, UK.
  • Yakunin AFDepartment of Chemical Engineering and Applied Chemistry, University of Toronto, Toronto, Canada.
  • Yan WArbor Biotechnologies, Cambridge, MA, USA.
  • Zhang FBroad Institute of MIT and Harvard, Cambridge, MA, USA.
  • Garrett RAArchaea Centre, Department of Biology, Copenhagen University, Copenhagen, Denmark.
  • Backofen RBioinformatics group, Department of Computer Science, University of Freiberg, Freiberg, Germany.
  • van der Oost JLaboratory of Microbiology, Wageningen University, Wageningen, The Netherlands.
  • Barrangou RDepartment of Food, Bioprocessing, and Nutrition Sciences, North Carolina State University, Raleigh, NC, USA.
  • Koonin EVNational Center for Biotechnology Information, National Library of Medicine, Bethesda, MD, USA. koonin@ncbi.nlm.nih.gov.
15 read1 want to readRigor 3.1Novelty 3.4Impact 2.7Reprod. 2.9

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Tariq Sato

Rigor 2/5Novelty 1/5Impact 4/5Reprod. 2/5

Great figures and clear story.

0

Eva Brown

Rigor 5/5Novelty 4/5Impact 2/5Reprod. 3/5

Nice but incremental.

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Sam Cohen

Rigor 2/5Novelty 4/5Impact 3/5Reprod. 5/5

Solid methods, convincing results.

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Lena Silva

Rigor 3/5Novelty 3/5Impact 2/5Reprod. 3/5

Interesting idea, small sample.