Nat Biotechnol · 2013

DNA targeting specificity of RNA-guided Cas9 nucleases

Hsu PD, Scott DA, Weinstein JA, Ran FA, Konermann S, Agarwala V, Li Y, Fine EJ, Wu X, Shalem O, Cradick TJ, Marraffini LA, Bao G, Zhang F.

Show affiliations
  • Hsu PD1] Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA. [2] McGovern Institute for Brain Research, Department of Brain and Cognitive Sciences, Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA. [3] Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts, USA. [4].
56 read5 want to readRigor 3.4Novelty 3.1Impact 2.9Reprod. 3.2

Most-cited papers citing this

  • Science · 2026 · cited by 10
  • Nat Cell Biol · 2025 · cited by 7
  • Chem Soc Rev · 2026 · cited by 4
  • Front Neurol · 2025 · cited by 3
  • Cancer Discov · 2025 · cited by 3

Log in to like, log, and share thoughts on this paper.

Discussion

Share your thoughts above to start a thread. Constructive feedback only — reply to and like others’ posts.

0

Ravi Rossi

Rigor 3/5Novelty 5/5Impact 5/5Reprod. 5/5

Replication is unclear to me.

0

Eva Wang

Rigor 5/5Novelty 4/5Impact 3/5Reprod. 3/5

Solid methods, convincing results.

0

Mei Garcia

Rigor 5/5Novelty 5/5Impact 5/5Reprod. 2/5

Solid methods, convincing results.

0

Maya Smith

Rigor 5/5Novelty 1/5Impact 2/5Reprod. 5/5

Important contribution to the field.

0

Eva Silva

Rigor 2/5Novelty 2/5Impact 5/5Reprod. 4/5

Important contribution to the field.

0

Ken Sato

Rigor 2/5Novelty 4/5Impact 3/5Reprod. 3/5

Replication is unclear to me.

0

Mei Brown

Rigor 3/5Novelty 5/5Impact 5/5Reprod. 2/5

Solid methods, convincing results.

0

Tariq Kim

Rigor 5/5Novelty 3/5Impact 1/5Reprod. 2/5

Interesting idea, small sample.

0

Yuki Wang

Rigor 2/5Novelty 5/5Impact 4/5Reprod. 4/5

Important contribution to the field.

0

Noor Smith

Rigor 3/5Novelty 3/5Impact 2/5Reprod. 4/5

Nice but incremental.

0

Eva Smith

Rigor 1/5Novelty 2/5Impact 2/5Reprod. 5/5

Solid methods, convincing results.