2026

Comprehensive repertoire of the chromosomal alteration and mutational signatures across 16 cancer types

Everall A, Tapinos A, Hawari A, Cornish AJ, Sud A, Chubb D, Kinnersley B, Frangou A, Barquin M, Jung J, Church DN, Alexandrov LB, Houlston RS, Gruber AJ, Wedge DC.

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  • Everall ADivision of Genetics and Epidemiology, The Institute of Cancer Research, London, UK.
  • Tapinos AManchester Cancer Research Centre, University of Manchester, Manchester, UK.
  • Hawari AManchester Cancer Research Centre, University of Manchester, Manchester, UK.
  • Cornish AJDivision of Genetics and Epidemiology, The Institute of Cancer Research, London, UK.
  • Sud ADivision of Genetics and Epidemiology, The Institute of Cancer Research, London, UK.
  • Chubb DDivision of Genetics and Epidemiology, The Institute of Cancer Research, London, UK.
  • Kinnersley BDivision of Genetics and Epidemiology, The Institute of Cancer Research, London, UK.
  • Frangou ANuffield Department of Medicine, Big Data Institute, University of Oxford, Oxford, UK.
  • Barquin MDepartment of Biology, University of Konstanz, Konstanz, Germany.
  • Jung JDepartment of Neurosurgery, King's College Hospital NHS Foundation Trust, London, UK.
  • Church DNOxford NIHR Comprehensive Biomedical Research Centre, Oxford University Hospitals NHS Foundation Trust, Oxford, UK.
  • Alexandrov LBDepartment of Cellular and Molecular Medicine, University of California, San Diego, La Jolla, CA, USA.
  • Houlston RSDivision of Genetics and Epidemiology, The Institute of Cancer Research, London, UK. richard.houlston@icr.ac.uk.
  • Gruber AJDepartment of Biology, University of Konstanz, Konstanz, Germany. gruber@uni-konstanz.de.
  • Wedge DCManchester Cancer Research Centre, University of Manchester, Manchester, UK. david.wedge@manchester.ac.uk.
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Whole-genome sequencing (WGS) enables exploration of the full spectrum of oncogenic processes that generate characteristic patterns of mutations. Mutational signatures provide clues to tumor etiology and highlight potentially targetable pathway defects. Here alongside single-base substitution, doublet-base substitution, small insertion and deletion and copy number aberration signatures previously covered by the Catalogue of Somatic Mutations in Cancer (COSMIC), we report signatures from an additional mutation type, structural variations (SVs), extracted de novo from WGS in 10,983 patients across 16 tumor types recruited to the 100,000 Genomes Project. Across the five mutation classes, we report 134 signatures, 26 of which are new to COSMIC, including an SV signature reference set. By relating signatures to genomic features and clinical phenotypes, we provide further insights into mutagenic processes and the application of signature analysis to precision oncology.

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