Nat Protoc · 2013

De novo transcript sequence reconstruction from RNA-seq using the Trinity platform for reference generation and analysis

Haas BJ, Papanicolaou A, Yassour M, Grabherr M, Blood PD, Bowden J, Couger MB, Eccles D, Li B, Lieber M, MacManes MD, Ott M, Orvis J, Pochet N, Strozzi F, Weeks N, Westerman R, William T, Dewey CN, Henschel R, LeDuc RD, Friedman N, Regev A.

Show affiliations
  • Haas BJBroad Institute of MIT and Harvard, 7 Cambridge Center, Cambridge, MA, 02142, USA.
  • Papanicolaou ACSIRO Ecosystem Sciences, Black Mountain Labs, Canberra, ACT 2601, Australia.
  • Yassour MBroad Institute of MIT and Harvard, 7 Cambridge Center, Cambridge, MA, 02142, USA.
  • Grabherr MScience for Life Laboratory, Department of Medical Biochemistry and Microbiology, Uppsala University, Uppsala, Sweden.
  • Blood PDPittsburgh Supercomputing Center, Carnegie Mellon University, Pittsburgh, PA, 15213, USA.
  • Bowden JCSIRO Information Management & Technology, 306 Carmody Rd, St Lucia QLD 4067, Australia.
  • Couger MBDepartment of Microbiology and Molecular Genetics, Oklahoma State University, USA.
  • Eccles DGenomics Research Centre, Griffith University, Gold Coast Campus, Qld 4222, Australia.
  • Li BDepartment of Computer Sciences, University of Wisconsin, Madison, WI, 53706, USA.
  • Lieber MTechnische Universität Dresden, Dresden, Saxony 01062, Germany.
  • MacManes MDUniversity of California, Berkeley and California Institute for Quantitative Biosciences Berkeley, CA 94720, USA.
  • Ott MCSIRO Ecosystem Sciences, Black Mountain Labs, Canberra, ACT 2601, Australia.
  • Orvis JInstitute for Genome Sciences, Baltimore, MD, 21201, USA.
  • Pochet NBroad Institute of MIT and Harvard, 7 Cambridge Center, Cambridge, MA, 02142, USA.
  • Strozzi FParco Tecnologico Padano, Loc. Cascina Codazza, 26900 Lodi, Italy.
  • Weeks NCorn Insects and Crop Genetics Research Unit, United States Department of Agriculture--Agricultural Research Service, Ames, IA 50011, USA.
  • Westerman RGenomics facility, Purdue University, West Lafayette, IN, 47907, USA.
  • William TGWT-TUD GmbH, Blasewitzer Strasse 43, Dresden, Saxony 01307, Germany.
  • Dewey CNDepartment of Computer Sciences, University of Wisconsin, Madison, WI, 53706, USA.
  • Henschel RIndiana University, 2709 East 10th Street, Bloomington, IN 47408,USA.
  • LeDuc RDIndiana University, 2709 East 10th Street, Bloomington, IN 47408,USA.
  • Friedman NThe Selim and Rachel Benin School of Computer Science, The Hebrew University of Jerusalem, Jerusalem 91904, Israel.
  • Regev ABroad Institute of MIT and Harvard, 7 Cambridge Center, Cambridge, MA, 02142, USA.
17 read1 want to readRigor 2.6Novelty 3.5Impact 2.8Reprod. 3.8

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Jonas Sato

Rigor 5/5Novelty 5/5Impact 1/5Reprod. 4/5

Important contribution to the field.

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Nadia Wang

Rigor 4/5Novelty 3/5Impact 3/5Reprod. 1/5

Solid methods, convincing results.